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Ribozymes

Methods and Protocols

  • Robert J Scarborough
  • Anne Gatignol
Book

Part of the Methods in Molecular Biology book series (MIMB, volume 2167)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Ribozyme Function

    1. Front Matter
      Pages 1-1
    2. Félix LaRoche-Johnston, Caroline Monat, Benoit Cousineau
      Pages 3-11
  3. Ribozyme Identification and Characterization

    1. Front Matter
      Pages 25-25
    2. Dibyajnan Chakraborty, Sagarmoy Ghosh
      Pages 45-59
    3. Sabrine Najeh, Kasra Zandi, Samia Djerroud, Nawwaf Kharma, Jonathan Perreault
      Pages 91-111
    4. Mohammad Kayedkhordeh, Ryota Yamagami, Philip C. Bevilacqua, David H. Mathews
      Pages 113-143
  4. Ribozyme Structure

    1. Front Matter
      Pages 145-145
    2. Charles G. Hoogstraten, Montserrat Terrazas, Anna Aviñó, Neil A. White, Minako Sumita
      Pages 183-202
  5. Ribozyme Conjugations

    1. Front Matter
      Pages 203-203
    2. Ryan P. Goguen, Anne Gatignol, Robert J. Scarborough
      Pages 253-267
  6. Ribozyme as Tools to Study Other RNAs

    1. Front Matter
      Pages 269-269
    2. Max Pietschmann, Gregor Tempel, Maral Halladjian, Nicolai Krogh, Henrik Nielsen
      Pages 271-285
    3. Alex C. Tuck, Marc Bühler
      Pages 287-301
  7. Back Matter
    Pages 303-304

About this book

Introduction

This volume provides protocols designed to study the function and the structure of diverse ribozymes. Chapters guide readers through different techniques to identify and characterize new ribozymes and methods to use ribozymes to alter the function of CRISPR-based guide RNAs, AgoshRNAs and aptamers or to study RNA capping and long non-coding RNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Ribozymes: Methods and Protocols aims to help in accelerating ribozyme research and inspiring others to develop new methods to study ribozyme structure and function.

Keywords

hammerhead ribozymes human microbiome archaeal genomes comparative genomics RNase P

Editors and affiliations

  • Robert J Scarborough
    • 1
  • Anne Gatignol
    • 2
  1. 1.Department of Microbiology and ImmunologyLady Davis Institute for Medical ResearchMontréalCanada
  2. 2.Department of Microbiology and ImmunologyLady Davis Institute for Medical ResearchMontréalCanada

Bibliographic information

  • DOI http://doi.org/10.1007/978-1-0716-0716-9
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2021
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-0716-0715-2
  • Online ISBN 978-1-0716-0716-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site