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Ribozymes pp 171-182 | Cite as

SHAPE Profiling to Probe Group II Intron Conformational Dynamics During Splicing

  • Timothy Wiryaman
  • Navtej ToorEmail author
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Part of the Methods in Molecular Biology book series (MIMB, volume 2167)

Abstract

手机体育投注平台Selective 2′-hydroxyl acylation analyzed by primer extension (SHAPE) is a widely used technique for studying the structure and function of RNA molecules. It characterizes the flexibility of single nucleotides in the context of the local RNA structure. Here we describe the application of SHAPE-MaP (mutational profiling) to study different conformational states of the group II intron during the self-splicing reaction.

Key words

RNA structure SHAPE-MaP Group II intron Chemical structure probing Next-generation sequencing 

Notes

Acknowledgement

This work was supported by NIH grant 1R01GM123275 awarded to N.T. T.W. was supported by the UCSD Cell, Molecular, and Genetics Training Program funded by NIH predoctoral training grant 5T32GM007240. Sequencing was conducted at the IGM Genomics Center, University of California, San Diego, La Jolla, CA. Dr. Kristen Jepsen and the IGM staff made helpful suggestions for library preparation and sequencing.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2021

Authors and Affiliations

  1. 1.Department of Chemistry and BiochemistryUniversity of California, San DiegoLa JollaUSA

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