Ribozymes pp 45-59 | Cite as

Demonstration of a Ribozyme in Epsilon Domain of Hepatitis B Virus RNA

  • Dibyajnan Chakraborty
  • Sagarmoy GhoshEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2167)


The epsilon domain of Hepatitis B virus plays a crucial role in encapsidation of viral pregenomic RNA and its partial NMR structure has been determined. However, we recently described a potassium-dependent ribonucleolytic activity associated with this region, so that a 53 nt long RNA containing the epsilon domain could release itself and cleaved other RNAs. We describe here the experimental methodologies for setting up the reactions and outline a general strategy for initial demonstration of this self-cleaving ribozyme activity.

Key words

HBV Epsilon Ribozyme Potassium 



S.G. acknowledges partial support of this work by Department of Science and Technology (Government of India) grant no. SR/FT/L-100/2004. Department of Biotechnology-BUILDER facility in University of Calcutta was used for Phosphorimager analysis. D.C. was supported by research fellowships from CSIR (9/28(664)/2006 EMR-I) and DBT (BT/01/CEIB/09/VI/10), Govt. of India.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2021

Authors and Affiliations

  1. 1.Dr. KPC Life Sciences Pvt. Ltd.KolkataIndia
  2. 2.Department of MicrobiologyUniversity of Calcutta, University College of Science and TechnologyKolkataIndia

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