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Ribozymes pp 79-89 | Cite as

Characterization and Optimization of a Deoxyribozyme with a Short Left Binding Arm

  • Yueyao Wang
  • Hanyang YuEmail author
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Part of the Methods in Molecular Biology book series (MIMB, volume 2167)

Abstract

手机体育投注平台Deoxyribozymes capable of catalyzing sequence-specific RNA cleavage have broad applications in biotechnology. In vitro selected RNA-cleaving deoxyribozymes normally contain two substrate-binding arms and a central catalytic core region. Here, we describe the systematic characterization and optimization of an RNA-cleaving deoxyribozyme with an unusually short left binding arm, and its special sequence requirement for its optimal catalytic activity.

Key words

Deoxyribozyme Catalytic DNA Nucleic acid enzyme RNA cleavage RNA transesterification Substrate-binding arm Cleavage site selectivity Metal ion catalysis 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2021

Authors and Affiliations

  1. 1.Department of Biomedical Engineering, College of Engineering and Applied SciencesNanjing UniversityNanjingChina

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