Substitutional RNA Editing in Plant Organelles

  • Mizuho IchinoseEmail author
  • Mamoru Sugita
Part of the Methods in Molecular Biology book series (MIMB, volume 2181)


RNA editing by cytidine (C) to uridine (U) conversions frequently occurs in land plant mitochondria and plastids. Target cytidines are specifically recognized by nuclear-encoded pentatricopeptide repeat (PPR) proteins in a sequence-specific manner. In the moss Physcomitrella patens, all PPR editing factors possess the DYW-deaminase domain at the C-terminus. Here, we describe methods for the direct sequencing of cDNA to detect RNA editing events and the RNA electrophoresis mobility shift assay (REMSA) to analyze the specific binding of PPR editing factors to their target RNA.

Key words

C-to-U editing Mitochondria Pentatricopeptide repeat Physcomitrella patens Plastid RNA-protein interaction 


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© Springer Science+Business Media, LLC, part of Springer Nature 2021

Authors and Affiliations

  1. 1.Center for Gene Research, Nagoya UniversityNagoyaJapan
  2. 2.Institute of Transformative Bio-Molecules (ITbM), Nagoya UniversityNagoyaJapan

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