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RNA Editing pp 83-95 | Cite as

Adenosine-to-Inosine RNA Editing Enzyme ADAR and microRNAs

  • Kang Yuting
  • Dan Ding
  • Hisashi IizasaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2181)

Abstract

RNA is subjected to over 100 different types of chemical modifications inside the cell. These modifications have various effects on its function and expression, resulting in RNA diversity. RNA editing or conversion of adenosine to inosine (A-to-I) in a double-stranded RNA is a type of RNA modification that can introduce mutations into the precursor of microRNA (miRNA). It can also regulate miRNA processing independently of A-to-I RNA editing. This chapter outlines the role of an A-to-I RNA editing enzyme ADAR in miRNA processing and the experimental systems used to analyze the interaction between miRNAs and ADAR.

Key words

ADAR A-to-I RNA editing miRNA Processing 

Notes

Acknowledgment

This text was prepared using the Ministry of Education, Culture, Sports, Science and Technology’s Scientific Research Fund. The drawing was made with the assistance of Ms. Sayuri Hamada.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2021

Authors and Affiliations

  1. 1.Department of Microbiology, Shimane University Faculty of MedicineIzumoJapan
  2. 2.Ningxia Medical UniversityYinchuanChina

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