手机体育投注平台

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    The Fundamental Contribution of Judah Folkman in the Setting of Angiogenesis Assays

    手机体育投注平台 Judah Folkman (1933–2008) made seminal discoveries on the mechanisms of angiogenesis which have opened a field of investigation worldwide. This chapter summarizes the fundamental contribution of Folkman in the...

    Domenico Ribatti in Vascular Morphogenesis (2021)

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    Dynamic Imaging of Mouse Embryos and Cardiac Development in Static Culture

    手机体育投注平台 Dynamic imaging is a powerful approach to assess the function of a developing organ system. The heart is a dynamic organ that undergoes quick morphological and mechanical changes through early embryonic develo...

    Andrew L. Lopez III, Irina V. Larina in Vascular Morphogenesis (2021)

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    RNA Editing in Neurological and Neurodegenerative Disorders

    手机体育投注平台 The brain is one of the organs that are preferentially targeted by adenosine-to-inosine (A-to-I) RNA editing, a posttranscriptional modification. This chemical modification affects neuronal development and fun...

    Pedro Henrique Costa Cruz, Yukio Kawahara in RNA Editing (2021)

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    In Vitro Coculture Assays of Angiogenesis

    手机体育投注平台 During angiogenesis, endothelial cells must undergo a coordinated set of morphological changes in order to form a new vessel. There is a need for endothelial cells to communicate with each other in order to ta...

    Haoche Wei, Ananthalakshmy Sundararaman in Vascular Morphogenesis (2021)

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    Quantification of Tumor Vasculature by Analysis of Amount and Spatial Dispersion of Caliber-Classified Vessels

    This protocol focuses on the quantitative description of the angioarchitecture of experimental tumor xenografts. This semiautomatic analysis is carried out on functional vessels and microvessels acquired by co...

    Marco Righi, Marco Presta, Arianna Giacomini in Vascular Morphogenesis (2021)

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    Substitutional RNA Editing in Plant Organelles

    手机体育投注平台 RNA editing by cytidine (C) to uridine (U) conversions frequently occurs in land plant mitochondria and plastids. Target cytidines are specifically recognized by nuclear-encoded pentatricopeptide repeat (PPR) ...

    Mizuho Ichinose, Mamoru Sugita in RNA Editing (2021)

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    手机体育投注平台 Protocol

    Microfluidic Device Setting by Coculturing Endothelial Cells and Mesenchymal Stem Cells

    The construction of vascular networks is essential for developing functional organ/tissue constructs in terms of oxygen and nutrient supply. Although recent advances in microfluidic techniques have allowed for...

    Masafumi Watanabe, Ryo Sudo in Vascular Morphogenesis (2021)

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    In Vivo Vascular Network Forming Assay

    The capability of forming functional blood vessel networks is critical for the characterization of endothelial cells. In this chapter, we will review a modified in vivo vascular network forming assay by replac...

    Hwan D. Kim, Ruei-Zeng Lin, Juan M. Melero-Martin in Vascular Morphogenesis (2021)

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    Discovering RNA Editing Events in Fungi

    手机体育投注平台 RNA editing is an important posttranscriptional process that alters the genetic information of RNA encoded by genomic DNA. Adenosine-to-inosine (A-to-I) editing is the most prevalent type of RNA editing in ani...

    Huiquan Liu, Jin-Rong Xu in RNA Editing (2021)

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    Studying Angiogenesis in the Rabbit Corneal Pocket Assay

    手机体育投注平台 The rabbit corneal micropocket assay uses the avascular cornea as a substrate to study angiogenesis in vivo. The continuous monitoring of neovascular growth in the same animal allows for the evaluation of drug...

    Lucia Morbidelli, Valerio Ciccone, Marina Ziche in Vascular Morphogenesis (2021)

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    By the Skin of Your Teeth: A Subcutaneous Mouse Model to Study Pulp Regeneration

    手机体育投注平台 Exiting developments in tissue engineering and new insights in stem cell biology have led to new possible strategies for the regeneration of damaged tissues in the oral cavity. The regeneration of the pulp–den...

    Annelies Bronckaers, Petra Hilkens, Esther Wolfs, Ivo Lambrichts in Vascular Morphogenesis (2021)

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    Live-Cell Quantification of APOBEC1-Mediated RNA Editing: A Comparison of RNA Editing Assays

    手机体育投注平台 APOBEC1 is a member of the AID/APOBECs, a group of deaminases responsible for the editing of C>U in both DNA and RNA. APOBEC1 is physiologically involved in C>U RNA editing: while hundreds of targets have been...

    Martina Chieca, Serena Torrini, Silvestro G. Conticello in RNA Editing (2021)

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    Conjugation as a Highly Sensitive Assay to Study Group II Intron Splicing In Vivo

    手机体育投注平台 Group II introns are noncoding sequences that interrupt genes, and that must be removed or spliced-out at the RNA level during gene expression. Following the transcription of interrupted genes, group II intron...

    Félix LaRoche-Johnston, Caroline Monat, Benoit Cousineau in Ribozymes (2021)

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    Quantitative Analysis of Adenosine-to-Inosine RNA Editing

    The conversion of adenosine to inosine (A to I) by RNA editing represents a common posttranscriptional mechanism for diversification of both the transcriptome and proteome, and is a part of the cellular respon...

    Turnee N. Malik, Jean-Philippe Cartailler, Ronald B. Emeson in RNA Editing (2021)

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    Cloning and Detection of Genomic Retrozymes and Their circRNA Intermediates

    Retrozymes are a novel family of non-autonomous retrotransposable elements that contain hammerhead ribozyme motifs. These retroelements are found widespread in eukaryotic genomes, with active copies present in...

    Amelia Cervera, Marcos de la Peña in Ribozymes (2021)

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    ALU A-to-I RNA Editing: Millions of Sites and Many Open Questions

    手机体育投注平台 Alu elements are repetitive short interspersed elements prevalent in the primate genome. These repeats account for over 10% of the genome with more than a million highly similar copies. A direct outcome of thi...

    Amos A. Schaffer, Erez Y. Levanon in RNA Editing (2021)

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    In Vitro Selection of Varkud Satellite Ribozyme Variants that Cleave a Modified Stem-Loop Substrate

    In vitro selection is an established approach to create artificial ribozymes with defined activities or to modify the properties of naturally occurring ribozymes. For the Varkud satellite ribozyme of Neurospora, ...

    Pierre Dagenais, Pascale Legault in Ribozymes (2021)

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    Computer-Aided Design of Active Pseudoknotted Hammerhead Ribozymes

    手机体育投注平台 Pseudoknots are important motifs for stabilizing the structure of functional RNAs. As an example, pseudoknotted hammerhead ribozymes are highly active compared to minimal ribozymes. The design of new RNA seque...

    Sabrine Najeh, Kasra Zandi, Samia Djerroud, Nawwaf Kharma, Jonathan Perreault in Ribozymes (2021)

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    Dynamics-Function Analysis in Catalytic RNA Using NMR Spin Relaxation and Conformationally Restricted Nucleotides

    A full understanding of biomolecular function requires an analysis of both the dynamic properties of the system of interest and the identification of those dynamics that are required for function. We describe ...

    Charles G. Hoogstraten, Montserrat Terrazas, Anna Aviñó, Neil A. White in Ribozymes (2021)

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    Using an L7Ae-Tethered, Hydroxyl Radical-Mediated Footprinting Strategy to Identify and Validate Kink-Turns in RNAs

    Kink-turns are important RNA structural modules that facilitate long-range tertiary interactions and form binding sites for members of the L7Ae family of proteins. Present in a wide variety of functional RNAs,...

    Stella M. Lai, Venkat Gopalan in Ribozymes (2021)

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