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    Principles of Spheroid Preparation for Creation of 3D Cardiac Tissue Using Biomaterial-Free Bioprinting

    Biomaterial-free three-dimensional (3D) bioprinting is a relatively new field within 3D bioprinting, where 3D tissues are created from the fusion of 3D multicellular spheroids, without requiring biomaterial. T...

    Chin Siang Ong, Isaree Pitaktong, Narutoshi Hibino in 3D Bioprinting (2020)

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    Microscale Thermophoresis (MST ) as a Tool for Measuring Dynamin Superfamily Protein (DSP)–Lipid Interactions

    Microscale thermophoresis (MST ) is a robust new fluorescence-based technology that enables measurement of biomolecular interactions and binding affinities (KD手机体育投注平台). is an immobilization-free alternative to surface...

    Nikhil Bharambe, Rajesh Ramachandran in Dynamin Superfamily GTPases (2020)

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    ambr® 15 Microbioreactors for CHO Cell Cultivation

    手机体育投注平台 The ambr 15 has become the industry’s standard automated microbioreactor system for mammalian cell culture. It has applications throughout the industry, most commonly for cell line screening and media/feed dev...

    Steve R. C. Warr in Animal Cell Biotechnology (2020)

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    Quantum Dots-Based Lateral Flow Test Strip for Glutathione Detection

    Lateral flow test strips (LFTSs) are particularly attractive for assaying targets due to their ability for fast response, simple equipment, portability, and straightforward signal readout. In this work, we des...

    Zhaohui Li, Juanzu Liu, Lin Zhang, Jianjun Li in Quantum Dots (2020)

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    Bioprinting for Skin

    We describe an extrusion-based method to print a human bilayered skin using bioinks containing human plasma and primary human fibroblasts and keratinocytes from skin biopsies. We generate 100 cm2手机体育投注平台 of printed skin ...

    Cristina Quílez, Gonzalo de Aranda Izuzquiza, Marta García in 3D Bioprinting (2020)

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    Measurement of Fluorescence for Monitoring Algal Growth and Health

    Measuring fluorescence is a noninvasive, inexpensive, and quick way of determining biomass concentration and health of the algae. Fluorescence is generally positively correlated with chlorophyll a手机体育投注平台 and can as such...

    Kristian Spilling, Jukka Seppälä in Biofuels from Algae (2020)

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    Analysis of Mitochondrial Membrane Fusion GTPase OPA1 Expressed by the Silkworm Expression System

    Mitochondria are highly dynamic organelles, which move and fuse to regulate their shape, size, and fundamental function. The dynamin-related GTPases play a critical role in mitochondrial membrane fusion. In vi...

    Tadato Ban, Naotada Ishihara in Dynamin Superfamily GTPases (2020)

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    Correction to: Determining Inorganic and Organic Phosphorus

    There are three corrections for this chapter. On page 90, 12th line from top should read as ‘(see step 6) with ultrapure water (e.g., 0.1 ml’ instead of ‘(see step 6) with ultrapure water (e.g., 1 ml’.

    Jaana Koistinen, Mervi Sjöblom, Kristian Spilling in Biofuels from Algae (2020)

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    HEK293 Cell-Based Bioprocess Development at Bench Scale by Means of Online Monitoring in Shake Flasks (RAMOS and SFR)

    手机体育投注平台 The platforms for bioprocess development have been developed in parallel to the needs of the manufacturing industry of biopharmaceuticals, aiming to ensure the quality and safety of their products. In this sen...

    Tibor Anderlei, Michael V. Keebler, Jordi Joan Cairó in Animal Cell Biotechnology (2020)

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    Development of Optical Sensors Based on Quantum Dots Using Molecularly Imprinted Polymers for Determination of Prilocaine

    Optical sensors are analytical tools that able to provide analyte information. There are several ways to design optical sensors. This chapter presents an interesting optical sensor to detect prilocaine, a medi...

    Nafiseh Kazemifard, Ali A. Ensafi, Zeinab Saberi in Quantum Dots (2020)

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    Bioprinting Strategies for Secretory Epithelial Organoids

    手机体育投注平台 Novel three-dimensional (3D) biofabrication platforms can allow magnetic 3D bioprinting (M3DB) by using magnetic nanoparticles to tag cells and then spatially arrange them in 3D around magnet dots. Here, we re...

    Ganokon Urkasemsin, Sasitorn Rungarunlert, João N. Ferreira in 3D Bioprinting (2020)

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    Monitoring Microalgal Neutral Lipid Accumulation with Nile Red

    手机体育投注平台 The widely applied Nile red (NR) method allows near real-time monitoring of microalgal neutral lipid accumulation. When added to a culture sample, optimally, the fluorescent dye NR penetrates the microalgal ce...

    Katariina Natunen in Biofuels from Algae (2020)

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    Development of Mammalian Cell Perfusion Cultures at Lab Scale: From Orbitally Shaken Tubes to Benchtop Bioreactors

    This chapter introduces the necessary concepts to develop mammalian cell perfusion cultures for the expression of therapeutic proteins at lab scale. We highlight the operation of the orbitally shaken tubes and...

    Moritz Wolf, Massimo Morbidelli in Animal Cell Biotechnology (2020)

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    Quantum Dots for Assessment of Reactive Oxygen Species Accumulation During Chemotherapy and Radiotherapy

    Quantum dots (QDs) are semiconductor nanoparticles ranging in size from 2 to 10 nm. QDs are increasingly being developed for biomedical imaging, targeted drug delivery, and green energy technology. Here we des...

    Kimal Honour Djam, Bong Han Lee, Sindhuja Suresh, Andrew Edet Ekpenyong in Quantum Dots (2020)

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    History and Trends of 3D Bioprinting

    The field of bioprinting is rapidly evolving as researchers innovate and drive the field forward. This chapter provides a brief overview of the history of bioprinting from the first described printer system in...

    Patrick Thayer, Hector Martinez, Erik Gatenholm in 3D Bioprinting (2020)

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    Determining Inorganic and Organic Phosphorus

    Phosphorus (P) is a macronutrient for all microalgal species, and the main form of uptake is orthophosphate (PO4). In this chapter we present a colorimetric method for determining the PO4手机体育投注平台 concentration and dissol...

    Jaana Koistinen, Mervi Sjöblom, Kristian Spilling in Biofuels from Algae (2020)

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    Introduction to Mass Spectrometry-Based Proteomics

    手机体育投注平台 Mass spectrometry, a technology to determine the mass of ionized molecules and biomolecules, is increasingly applied for the global identification and quantification of proteins. Proteomics applies mass spectr...

    Rune Matthiesen, Jakob Bunkenborg in Mass Spectrometry Data Analysis in Proteomics (2020)

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    Computer-Aided Design and Manufacturing (CAD/CAM) for Bioprinting

    Three-dimensional (3D) printing of human tissues and organs has been an exciting area of research for almost three decades [Bonassar and Vacanti. J Cell Biochem. 72(Suppl 30–31):297–303 (1998)]. The primary go...

    Cormac D. Fay in 3D Bioprinting (2020)

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    Absolute and Relative Methods for Fluorescence Quantum Yield Evaluation of Quantum Dots

    Optical spectroscopy techniques are crucial for the evaluation and use of quantum dots (QDs) in life and materials science. In that context, the fluorescence quantum yield (Φf) is an essential parameter in the as...

    Renato E. de Araujo, Christian T. Dominguez in Quantum Dots (2020)

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    Total Carbohydrate Content Determination of Microalgal Biomass by Acid Hydrolysis Followed by Spectrophotometry or Liquid Chromatography

    The carbohydrate fraction of microalgal biomass is complex and consists of a combination of neutral, acidic, amino sugars, and sugar alcohols. These carbohydrate amalgamations can be difficult to accurately qu...

    S. Van Wychen, L. M. L. Laurens in Biofuels from Algae (2020)

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