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    Guidelines for the Optimization and Validation of In Situ Hybridization

    As RNA in situ hybridization (ISH) moves into the mainstream lab and increasingly into clinical adoption and additional multiplexing techniques are developed to enable further RNA ISH identification, a set of ...

    Julia Jones, William J. Howat in In Situ Hybridization Protocols (2020)

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    Expression Analysis of Important Genes Involved in Nitrogen Metabolism Under Hypoxia

    Hypoxia or anoxia condition can occurs during flooding or waterlogging and can cause intense damage to the plants. Since oxygen is important for active operation of electron transport chain in mitochondria to ...

    Mallesham Bulle, Reddy Kishorekumar, Aakanksha Wany in Nitrogen Metabolism in Plants (2020)

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    New Developments in Microwave–Assisted Solid Phase Peptide Synthesis

    The unique combination of microwave heating with optimized carbodiimide activation has proven to be an indispensable technique for high-throughput peptide production. Here, we describe new methods in microwave...

    Sandeep K. Singh, Jonathan M. Collins in Peptide Synthesis (2020)

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    Single Cell Analysis by High-Resolution Atmospheric-Pressure MALDI MS Imaging

    手机体育投注平台 Complementary to other imaging techniques, MALDI MS imaging provides label-free analysis and chemical specificity, covering a wide range of molecules that can be visualized simultaneously. Here, we highlight a...

    Dhaka Ram Bhandari, Giulia Coliva, Maria Fedorova in Single Cell Metabolism (2020)

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    Measuring Interactions Between Tau and Aggregation Inducers with Single-Molecule Förster Resonance Energy Transfer

    手机体育投注平台 Tau is an intrinsically disordered protein implicated in the pathogenesis of Alzheimer’s disease and other neurodegenerative disorders. Here we describe the application of single-molecule Förster resonance ene...

    Sanjula P. Wickramasinghe, Elizabeth Rhoades in Intrinsically Disordered Proteins (2020)

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    Analysis of Arbuscular Mycorrhizal Fungal Communities by Terminal Restriction Fragment Length Polymorphism (TRFLP)

    Terminal restriction fragment length polymorphism (TRFLP) approaches enable the detection and identification of microbial taxa into samples coming from root or soil material DNA extraction. The low taxonomic d...

    Álvaro López-García in Arbuscular Mycorrhizal Fungi (2020)

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    Diagnosis of Tropical Fascioliasis Using Specific Antigen Detection in Feces of Infected Human Being or Animal Species

    Tropical fascioliasis is considered to be a significant factor in limiting livestock production. The development of sustained strategies for accurate early diagnosis is essential for controlling Fasciola gigantic...

    Neelima Gupta, Said I. Shalaby, Dileep K. Gupta in Experimental Protocols in Biotechnology (2020)

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    A Guide for Protein–Protein Docking Using SwarmDock

    Many of the biological functions of the cell are driven by protein–protein interactions. However, determining which proteins interact and exactly how they do so to enable their functions, remain major research...

    Iain H. Moal, Raphael A. G. Chaleil, Mieczyslaw Torchala in Protein Structure Prediction (2020)

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    Targeting an Intrinsically Disordered Protein by Covalent Modification

    手机体育投注平台 Intrinsically disordered proteins (IDPs) play important roles in the regulation of cellular function and in disease, and thus they represent an important group of therapeutic targets. Yet, members of this “dis...

    Hung Huy Nguyen, Péter Ábrányi-Balogh, László Petri in Intrinsically Disordered Proteins (2020)

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    Transfection of Small Noncoding RNAs into Arabidopsis thaliana Protoplasts

    手机体育投注平台 Polyethylene glycol transfection of plant protoplasts represents an efficient method to incorporate foreign DNA and study transient gene expression. Here, we describe an optimized protocol to deliver small non...

    Stéphanie Lalande, Marjorie Chery, Elodie Ubrig, Guillaume Hummel in RNA Tagging (2020)

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    Step-by-Step Protocol for Superparamagnetic Nanoparticle-Based Endosome and Lysosome Isolation from Eukaryotic Cell

    Here, we report our step-by-step protocol for superparamagnetic nanoparticle (SPMNP)-based endosome and lysosome isolation from HeLa. Briefly, we synthesized SPMNP 1.0 with iron oxide (Fe3O4手机体育投注平台) core using thermal d...

    Deepak B. Thimiri Govinda Raj, Niamat Ali Khan in Stem Cell Nanotechnology (2020)

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    Avian Population Genomics Taking Off: Latest Findings and Future Prospects

    手机体育投注平台 Birds are one of the most recognizable and diverse groups of organisms on earth. This group has played an important role in many fields, including the development of methods in behavioral ecology and evolution...

    Kira E. Delmore, Miriam Liedvogel in Statistical Population Genomics (2020)

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    Transmission of Viruses by Whiteflies

    The whiteflies (Bemisia tabaci, Hemiptera: Aleyrodidae) are efficient vectors of many plant viruses (Begomovirus, Carlavirus, Crinivirus, Ipomovirus, Torradovirus). Whiteflies are known to transmit more than 80 v...

    Alangar Ishwara Bhat, Govind Pratap Rao in Characterization of Plant Viruses (2020)

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    Immunostaining and In Situ Hybridization of the Developing Acoel Nervous System

    The study of acoel morphologies has been recently stimulated by the knowledge that this group of animals represents an early offshoot of the Bilateria. Understanding how organ systems and tissues develop and t...

    Elena Perea-Atienza, Brenda Gavilán, Simon G. Sprecher, Pedro Martinez in Brain Development (2020)

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    Native Protein Mass Spectrometry

    In native mass spectrometry, non-covalent interactions are preserved in solution and through transfer to the gas phase. This technique can be used to characterize the composition, stoichiometry, and architectu...

    Timothy M. Allison, Mark T. Agasid in Protein Nanotechnology (2020)

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    Direct Binding Analysis Between C-Type Lectins and Glycans Using Immunoglobulin Receptor Fusion Proteins

    C-type lectins bind to carbohydrate structures in a Ca2+-dependent manner. Some transmembrane forms of lectins act as innate immune receptors and induce signal transduction pathways in macrophages and dendritic c...

    Miyuki Watanabe, Zakaria Omahdi, Sho Yamasaki in Lectin Purification and Analysis (2020)

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    Proteolistics: A Protein Delivery Method

    Intracellular protein delivery in plant tissues is becoming an important tool for addressing both basic and applied research questions by plant biologists, especially in the era of genome editing. The ability ...

    Susana Martin-Ortigosa, Kan Wang in Biolistic DNA Delivery in Plants (2020)

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    Analysis of Changes in Plant Cell Wall Composition and Structure During Cold Acclimation

    The cell wall has a crucial influence on the mechanical properties of plant cells. It therefore has a strong impact on the freezing behavior and very likely also the freezing tolerance of plants. However, not ...

    Daisuke Takahashi, Ellen Zuther, Dirk K. Hincha in Plant Cold Acclimation (2020)

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    Restricted Pollination for Tracing Individual Pollen Tubes in a Pistil

    As one of the essential steps to complete sexual reproduction, a pollen tube is precisely guided to an embryo sac to deliver the sperm cells. This ovule targeting by a pollen tube is one of the essential steps...

    Taro Takahashi, Toshiyuki Mori, Tomoko Igawa in Pollen and Pollen Tube Biology (2020)

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    Estimation and Imaging Techniques to Study Lipids in Mammalian Samples

    Lipids are small biomolecules that constitute structural elements of the cellular membranes and play crucial functions to maintain various cellular processes indispensable for survival. In general, there are f...

    Sudhanshu Shukla, Sanghamitra Mishra in Analysis of Membrane Lipids (2020)

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