手机体育投注平台

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  1. No Access手机体育投注平台

    Protocol

    In Vitro Drug Efficacy Testing Against Trypanosoma brucei

    The recent endorsement of fexinidazole by the European Medicines Agency for the treatment of human African trypanosomiasis has demonstrated the high predictive value of cell-based assays for parasite chemother...

    Marcel Kaiser, Pascal Mäser in Trypanosomatids (2020)

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    Protocol

    Bioluminescence Resonance Energy Transfer (BRET) Coupled Near-Infrared Imaging of Apoptotic Cells

    手机体育投注平台 Detection of apoptotic cells is crucial for understanding the mechanism of diseases and for therapy development. So far, visible-emitting fluorescent probes such as FITC-labeled Annexin V has been widely used ...

    Setsuko Tsuboi, Takashi Jin in Bioluminescent Imaging (2020)

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    Vascular-Related Biomarkers of Ischemic Stroke

    手机体育投注平台 Stroke remains a leading cause of death and disability worldwide, with ischemic stroke accounting for the vast majority of all stroke cases. Despite advances in the treatment of acute stroke with tissue-plasmi...

    Daniel Arteaga, Bradford B. Worrall in Stroke Biomarkers (2020)

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    手机体育投注平台 Protocol

    Using Lactococcus lactis as Surrogate Organism to Study Group A Streptococcus Surface Proteins

    手机体育投注平台 The isolation of a single Group A Streptococcus (GAS) virulence determinant in functional investigations is challenging, as GAS employs a multitude of virulence factors. The redundancy between many surface pro...

    Catherine (Jia-Yun) Tsai, Jacelyn M. S. Loh, Sam Blanchett in Group A Streptococcus (2020)

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    手机体育投注平台 Protocol

    In Vivo Models for Pathotyping and Vaccine Efficacy for Swine Influenza

    Swine influenza is a disease of the respiratory tract caused by influenza A virus (IAV). Experimental inoculation of pigs involves either aerosolization of virus and inhalation or the direct introduction of vi...

    Kelly M. Lager, Amy L. Vincent in Animal Influenza Virus (2020)

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    CRISPR/Cas9 Technology Applied to the Study of Proteins Involved in Calcium Signaling in Trypanosoma cruzi

    Chagas disease is a vector-borne tropical disease affecting millions of people worldwide, for which there is no vaccine or satisfactory treatment available. It is caused by the protozoan parasite Trypanosoma cruz...

    Noelia Lander, Miguel A. Chiurillo, Roberto Docampo in Trypanosomatids (2020)

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    Oligonucleotide Enrichment of HSV-1 Genomic DNA from Clinical Specimens for Use in High-Throughput Sequencing

    手机体育投注平台 To date more than 400 genomes of herpes simplex virus 1 (HSV-1) and the distantly related HSV-2 have been examined using deep sequencing techniques. This powerful approach has been especially useful for reveal...

    Mackenzie M. Shipley, Molly M. Rathbun, Moriah L. Szpara in Herpes Simplex Virus (2020)

  8. Protocol

    In Vivo Bioluminescence Imaging to Assess Compound Efficacy Against Trypanosoma brucei

    Traditional animal models for human African trypanosomiasis rely on detecting Trypanosoma brucei brucei手机体育投注平台 parasitemia in the blood. Testing the efficacy of new compounds in these models is cumbersome because it may...

    Ryan Ritchie, Michael P. Barrett, Jeremy C. Mottram, Elmarie Myburgh in Trypanosomatids (2020)

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    Bioluminescent Imaging of Single Bacterial Cells Using an Enhanced ilux Operon

    The lux手机体育投注平台 operon is a useful reporter for bioluminescence imaging due to its independence of exogenous luciferin supply, but its relatively low brightness hampers the imaging of single cells. This chapter describes...

    Carola Gregor in Bioluminescent Imaging (2020)

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    Neuroplasticity Biomarkers in Experimental Stroke Recovery

    This chapter focuses on the main classes of plasticity biomarkers consisting various neurovascular specific regulators, glial/neuronal proteins, and growth factor profiles related to selective types of exper...

    Philip V. Peplow, Bridget Martinez, D. Mascareñas in Stroke Biomarkers (2020)

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    Detection of Fibronectin-Binding Proteins of Streptococcus pyogenes Using Ligand Blot Analysis

    Streptococcus pyogenes utilizes extracellular cellular matrix (ECM) proteins to adhere to human tissues and internalize into host cells. Fibronectin (Fn) is one of the most abundant ECM proteins and targeted by a...

    Masanobu Nakata, Shigetada Kawabata in Group A Streptococcus (2020)

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    Equine Influenza Diagnosis: Sample Collection and Transport

    In horses, presumptive diagnosis of equine influenza is commonly made on the basis of clinical signs. This alone is insufficient for confirmation of equine influenza, because other equine infectious respirator...

    Thomas M. Chambers, Stephanie E. Reedy in Animal Influenza Virus (2020)

  13. 手机体育投注平台 Protocol

    Next-Generation Analysis of Trypanosomatid Genome Stability and Instability

    手机体育投注平台 Understanding the rate and patterns of genome variation is becoming ever more amenable to whole-genome analysis through advances in DNA sequencing, which may, at least in some circumstances, have supplanted mo...

    Emma M. Briggs, Catarina A. Marques, Joao Reis-Cunha, Jennifer Black in Trypanosomatids (2020)

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    Equine Influenza Serological Methods

    Serologic tests for equine influenza virus (EIV) antibodies are used for many purposes, including retrospective diagnosis, subtyping of virus isolates, antigenic comparison of different virus strains, and meas...

    Thomas M. Chambers, Stephanie E. Reedy in Animal Influenza Virus (2020)

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    Phenotypic and Genotypic Testing of HSV-1 and HSV-2 Resistance to Antivirals

    手机体育投注平台 Resistance testing of antivirals to herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) can be done by phenotypic and genotypic methods. The determination of a resistant phenotype is based on the calculatio...

    Andreas Sauerbrei, Kathrin Bohn-Wippert in Herpes Simplex Virus (2020)

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    手机体育投注平台 Protocol

    In Vivo Bioluminescent Imaging of Yersinia ruckeri Pathogenesis in Fish

    Bioluminescent reporters and advanced luciferase technologies are useful to study host–pathogen interactions. This chapter describes the use of the luxCDABE operon from Photorhabdus luminescens手机体育投注平台 as a tool to analy...

    José A. Guijarro, Jessica Méndez in Bioluminescent Imaging (2020)

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    Blood Biomarkers for Stroke Differentiation

    Acute stroke is a neurological emergency. Time sensitive treatment decisions depend on the correct diagnosis of stroke and its subtype: ischemic or hemorrhagic. An early diagnosis of ischemic stroke is require...

    Deepti Vibha, Shubham Misra in Stroke Biomarkers (2020)

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    Protocol

    Dynamic Interactions of Group A Streptococcus with Host Macrophages

    手机体育投注平台 Macrophages play a critical role in Group A Streptococcus (GAS) recognition and the consequent activation of innate immunity and inflammatory responses against the pathogen. In parallel, GAS deploys several st...

    Angelica Montenegro Riestra, J. Andrés Valderrama in Group A Streptococcus (2020)

  19. No Access手机体育投注平台

    Protocol

    Type A Influenza Virus Detection from Horses by Real-Time RT-qPCR and Insulated Isothermal RT-PCR

    Equine influenza (EI) is a highly contagious disease of horses caused by the equine influenza virus (EIV) H3N8 subtype. EI is the most important respiratory virus infection of horses and can disrupt major eque...

    Udeni B. R. Balasuriya in Animal Influenza Virus (2020)

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    Identifying Trypanosome Protein–RNA Interactions Using RIP-Seq

    Trypanosomatids rely primarily on posttranscriptional mechanisms for the control of gene expression, with regulation of RNA processing, localization, degradation, and translation by RNA-binding proteins (RBPs)...

    Elisha Mugo, Esteban D. Erben in Trypanosomatids (2020)

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