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    Single Cell Analysis by High-Resolution Atmospheric-Pressure MALDI MS Imaging

    手机体育投注平台 Complementary to other imaging techniques, MALDI MS imaging provides label-free analysis and chemical specificity, covering a wide range of molecules that can be visualized simultaneously. Here, we highlight a...

    Dhaka Ram Bhandari, Giulia Coliva, Maria Fedorova in Single Cell Metabolism (2020)

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    High-Resolution Mapping of 3’ Extremities of RNA Exosome Substrates by 3’ RACE-Seq

    The main 3′-5′ exoribonucleolytic activity of eukaryotic cells is provided by the RNA exosome. The exosome is constituted by a core complex of nine subunits (Exo9), which coordinates the recruitment and the ac...

    Hélène Scheer, Caroline De Almeida, Natalia Sikorska in The Eukaryotic RNA Exosome (2020)

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    Ambient Lipidomic Analysis of Single Mammalian Oocytes and Preimplantation Embryos Using Desorption Electrospray Ionization (DESI) Mass Spectrometry

    Desorption electrospray ionization (DESI) is a spray-based ambient ionization method for mass spectrometry (MS) that generates ions in native atmospheric conditions (e.g., pressure and temperature). Ambient io...

    Christina R. Ferreira, Valentina Pirro, Alan K. Jarmusch in Single Cell Metabolism (2020)

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    Mouse Model of Alcoholic Steatohepatitis

    Alcoholic liver disease (ALD) is one of the most common causes of chronic liver disease in Western countries. The spectrum of ALD ranges from simple steatosis to steatohepatitis to cirrhosis and hepatocellular...

    Adeline Bertola in Kupffer Cells (2020)

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    手机体育投注平台 Protocol

    Sample Preparation of Optically Cleared Liver Tissue to Identify Liver Macrophages Using 3D Microscopy

    Histology, or the study of tissue microanatomy, is essential to understanding the in situ function of varying cell types within an organ. How cells are distributed throughout organs provides an indication of h...

    Otto Strauss, Niklas K. Björkström in Kupffer Cells (2020)

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    Assessment of Neutrophil Apoptosis

    手机体育投注平台 The process of neutrophil apoptosis has an important role in the resolution of acute inflammation. Apoptotic cell death is characterized by a coordinated sequence of cellular alterations that serve to uncouple...

    Nicole D. Barth, Marc Vendrell, David A. Dorward, Adriano G. Rossi in Neutrophil (2020)

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    手机体育投注平台 Protocol

    Photoprotective Activity Assay Toward Ultraviolet B in Human Keratinocytes

    手机体育投注平台 Design and discovery of novel sunscreens are of great importance to protect the human skin toward ultraviolet-induced damages because of the incidence of skin cancers that is believed to have increased dependi...

    Mehmet Varol in Epidermal Cells (2020)

  8. Protocol

    Correction to: Assessing Tissue-Specific Autophagy Flux in Adult Caenorhabditis elegans

    Correction to: Chapter 17 in: Sean P. Curran (ed.), Aging: Methods and Protocols, Methods in Molecular Biology, vol. 2144

    Jessica T. Chang, Malene Hansen, Caroline Kumsta in Aging (2020)

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    Contribution of Immunohistochemistry in Revealing S100A7/JAB1 Colocalization in Psoriatic Epidermal Keratinocyte

    The application of immunohistological methods provides an invaluable contribution in revealing the protein colocalization, which may reflect the occurrence of molecular interaction processes.

    Mariagrazia Granata, Evangelia Skarmoutsou, Maria Clorinda Mazzarino in Epidermal Cells (2020)

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    Metabolic Network Reconstructions to Predict Drug Targets and Off-Target Effects

    The drug development pipeline has stalled because of the difficulty in identifying new drug targets while minimizing off-target effects. Computational methods, such as the use of metabolic network reconstructi...

    Kristopher Rawls, Bonnie V. Dougherty in Metabolic Flux Analysis in Eukaryotic Cells (2020)

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    手机体育投注平台 Protocol

    Isolation, Culture, and Induction of Plasticity in ILC2s

    手机体育投注平台 Innate lymphoid cells (ILCs) are lymphocytes with critical roles in homeostasis, inflammation, and immunity to pathogens. ILCs are rare relative to other immune cell populations and are primarily defined by la...

    Jonathan Silver, Alison A. Humbles, Yoichiro Ohne in Innate Lymphoid Cells (2020)

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    Study of Caveolae-Dependent Mechanoprotection in Human Muscle Cells Using Micropatterning and Live-Cell Microscopy

    Caveolae are plasma membrane organelles that are, among many other features, involved in mechanosensing and mechanoprotection. Different tools have been developed to study caveolae-dependent mechanoprotection ...

    Melissa Dewulf, Cedric M. Blouin in Caveolae (2020)

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    手机体育投注平台 Protocol

    Testing the Specificity of Compounds Designed to Inhibit CPT1A in T Cells

    In response to antigen and costimulation, T cells undergo a series of metabolic transitions that fulfill the biosynthetic demands of clonal expansion, differentiation, and effector function. Following antigen ...

    Roddy S. O’Connor, Michael C. Milone in Cell Reprogramming for Immunotherapy (2020)

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    Generation of Knockout Gene-Edited Human Intestinal Organoids

    We discuss a methodology to generate and study knockout gene-edited human intestinal organoids. We describe the generation of knockout human embryonic stem cell lines that we then differentiate into mature hum...

    Chathruckan Rajendra, Tomas Wald, Kevin Barber, Jason R. Spence in Intestinal Stem Cells (2020)

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    Analyzing the Metabolism of Metastases in Mice

    手机体育投注平台 Metastasis formation is the leading cause of death in cancer patients. It has recently emerged that cancer cells adapt their metabolism to successfully transition through the metastatic cascade. Consequently, ...

    Patricia Altea-Manzano, Dorien Broekaert in Metabolic Flux Analysis in Eukaryotic Cells (2020)

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    Production of Lentivirus for the Establishment of CAR-T Cells

    One of the most versatile gene transfer methods involves the use of recombinant lentiviral vectors since they can transduce both dividing and nondividing cells, are considered to be safe and provide long-term ...

    Marlous G. Lana, Bryan E. Strauss in Chimeric Antigen Receptor T Cells (2020)

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    Human MAIT Cell Activation In Vitro

    手机体育投注平台 Mucosal-associated invariant T (MAIT) cells are an abundant innate-like T cell subset in humans, enriched in mucosal tissues and the liver. MAIT cells express a semi-invariant T cell receptor (TCR) and recogni...

    Joachim P. Hagel, Lucy C. Garner, Matthew Bilton, Hema Mehta, Tianqi Leng in MAIT Cells (2020)

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    Analysis of Endocytosis and Intracellular Trafficking of Boric Acid/Borate Transport Proteins in Arabidopsis

    Plants take up inorganic nutrients from the soil by transport proteins located in the plasma membrane of root cells. Boron (B) is an essential element for plant growth; it taken up and translocated by boric ac...

    Akira Yoshinari, Junpei Takano in Plant Endosomes (2020)

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    Identification of Murine Innate Lymphoid Cell Subsets in Barrier Tissues and Their Draining Lymph Nodes Using Flow Cytometry

    Innate lymphoid cells (ILCs) are present in most tissues within the body but have been most extensively studied within mucosal barriers such as the lung and intestine. Isolation of immune cells from such tissu...

    Emma E. Dutton, David R. Withers in Innate Lymphoid Cells (2020)

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    Concurrent Immunohistochemical Localization and Western Blot Analysis of the MIF Receptor, CD74, in Formalin-Fixed, Paraffin-Embedded Tissue

    Archived, formalin-fixed, paraffin-embedded tissue provides a robust resource for assessing protein expression in a variety of complex tissue types. Immunohistochemical localization techniques allow one to ide...

    Amanda Graham, Warren B. Nothnick in Macrophage Migration Inhibitory Factor (2020)

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