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    Immunostaining and In Situ Hybridization of the Developing Acoel Nervous System

    手机体育投注平台 The study of acoel morphologies has been recently stimulated by the knowledge that this group of animals represents an early offshoot of the Bilateria. Understanding how organ systems and tissues develop and t...

    Elena Perea-Atienza, Brenda Gavilán, Simon G. Sprecher, Pedro Martinez in Brain Development (2020)

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    A Novel Preclinical Rat Model of Alzheimer’s Disease

    手机体育投注平台 We have developed a novel rat model that correlates with seemingly normal individuals who are predisposed (at-risk) to developing Alzheimer’s disease (AD). This work summarizes the findings we have reported on...

    Karim A. Alkadhi in Basic Neurobiology Techniques (2020)

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    Trauma-Focused Cognitive Behavioral Therapy (TF-CBT)

    Trauma-focused cognitive behavioral therapy (TF-CBT) is currently considered by multiple sources to be the “gold standard” treatment for children and adolescents experiencing posttraumatic stress and associate...

    Brian Allen, Elizabeth Riden, Chad E. Shenk in Cognitive Behavioral Therapy in Youth: Tra… (2020)

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    Practical Guidelines for Two-Color SMLM of Synaptic Proteins in Cultured Neurons

    手机体育投注平台 The application of single-molecule localization microscopy (SMLM) to the study of synaptic proteins has shown that the postsynaptic density (PSD) is organized heterogeneously in subsynaptic domains (SSDs) that...

    Xiaojuan Yang, Christian G. Specht in Single Molecule Microscopy in Neurobiology (2020)

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    Ex Vivo Studies of Optic Nerve Axon Electrophysiology

    The use of ex vivo compound action potential (CAP) recordings from intact optic nerves is an ideal model to study white matter function without the influence of gray matter. Here, we describe how freshly disse...

    Chinthasagar Bastian, Sylvain Brunet, Selva Baltan in Axon Degeneration (2020)

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    Transforming FIB-SEM Systems for Large-Volume Connectomics and Cell Biology

    Isotropic high-resolution imaging of large volumes provides unprecedented opportunities to advance connectomics and cell biology research. Conventional focused ion beam scanning electron microscopy (FIB-SEM) o...

    C. Shan Xu, Song Pang, Kenneth J. Hayworth, Harald F. Hess in Volume Microscopy (2020)

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    Gene Silencing in Chicken Brain Development

    Despite the development of brain organoids and neural cultures derived from iPSCs (induced pluripotent stem cells), brain development can only be studied in an animal. The mouse is the most commonly used verte...

    Georgia Tsapara, Irwin Andermatt, Esther T. Stoeckli in Brain Development (2020)

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    Ultramicroscopy of Nerve Fibers and Neurons: Fine-Tuning the Light Sheets

    手机体育投注平台 Light sheet generation approaches employed in the ultramicroscopy imaging technique are presented, and three different designs are discussed. These include slit-based as well as more advanced slit-free setups,...

    Saiedeh Saghafi, Klaus Becker, Nina Jährling in Neurohistology and Imaging Techniques (2020)

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    Combining Transcranial Direct Current Stimulation and Electrophysiology to Understand the Memory Representations that Guide Attention

    How attention is used during visual search is intricately associated with memory. A considerable body of work has demonstrated that representations in both working memory and long-term memory can guide attenti...

    Shrey Grover, Robert M. G. Reinhart in Spatial Learning and Attention Guidance (2020)

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    Retinal Strip Culture for Studying Ganglion Cell Axon Growth

    Retinal ganglion cell (RGC) axons extend along the inner limiting membrane, which forms the extracellular matrix (ECM) containing laminin, collagen, and proteoglycans. RGC axons express integrin, which is acti...

    Masayuki Yamashita in Retinal Development (2020)

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    The Properties of Light Governing Biological Microscopy

    手机体育投注平台 Just as the telescope allowed us to view the heavens, the microscope has opened vistas within the microcosm of the natural world. Here we review the properties of light and its interaction with matter that gov...

    Pina Colarusso in Neurohistology and Imaging Techniques (2020)

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    Nonfluorescent RNA In Situ Hybridization Combined with Antibody Staining to Visualize Multiple Gene Expression Patterns in the Embryonic Brain of Drosophila

    In Drosophila, the brain arises from about 100 neural stem cells (called neuroblasts) per hemisphere which originate from the neuroectoderm. Products of developmental control genes are expressed in spatially rest...

    David Jussen, Rolf Urbach in Brain Development (2020)

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    Photometric Patch Electrode to Simultaneously Measure Neural Electrical Activity and Optical Signal in the Brain Tissue

    Photometric patch electrode (PME) measures optical signal of neurons simultaneously with electrical activity in deep brain tissues. As a light guide, PME transmits laser light to the tip of electrode to excite...

    Harunori Ohmori in Basic Neurobiology Techniques (2020)

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    Adapting Cognitive Behavioral Therapy for Children with Autism Spectrum Disorder and Comorbid Anxiety and Obsessive-Compulsive Disorders

    Research has demonstrated many children and adolescents with autism spectrum disorder (ASD) display symptoms of anxiety and often meet criteria for anxiety as well as obsessive-compulsive disorders (OCD). Howe...

    Kelly N. Banneyer, Rachel Fein in Cognitive Behavioral Therapy in Youth: Tra… (2020)

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    Postoperative Cognitive Function Following General Anesthesia in Children

    Research into the developmental effects of anesthetic use began from the study of the fetal and postnatal effects of maternal alcohol consumption. Broadly speaking, alcohol is an anesthetic of sorts, and its t...

    Maiko Satomoto in General Anesthesia Research (2020)

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    Measuring Bioenergetic Signatures of Peripheral Nerve Segments by Extracellular Flux Analysis

    Changes of energy metabolism in axons and their adjacent glia as well as alterations in metabolic axon–glia cross talk are emerging as central mechanistic components underlying axon degeneration. The analysis ...

    Bogdan Beirowski in Axon Degeneration (2020)

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    Forget About Electron Micrographs: A Novel Guide for Using 3D Models for Quantitative Analysis of Dense Reconstructions

    With the rapid evolvement in the automation of serial micrographs, acquiring fast and reliably giga- to terabytes of data is becoming increasingly common. Optical, or physical sectioning, and subsequent imagin...

    Daniya J. Boges, Marco Agus, Pierre Julius Magistretti, Corrado Calì in Volume Microscopy (2020)

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    Immunohistochemistry and RNA In Situ Hybridization in Mouse Brain Development

    During development, the mouse brain is progressively divided into functionally distinct compartments. Numerous neuronal and glial cell types are subsequently generated in response to various inductive signals....

    Jinling Liu, Aimin Liu in Brain Development (2020)

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    Consumer Versus Dedicated Digital Cameras in Photomicrography

    手机体育投注平台 A number of consumer digital cameras (compact, bridge, single lens reflex [SLR], and system ones) are of sufficiently high quality to qualify as suitable for photomicrography and represent an affordable altern...

    Jörg Piper, Radek Pelc in Neurohistology and Imaging Techniques (2020)

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    Multivariate Methods to Track the Spatiotemporal Profile of Feature-Based Attentional Selection Using EEG

    This chapter provides a tutorial style guide to analyzing electroencephalogram (EEG) data contingent on feature-based attentional selection. It is targeted at researchers that currently investigate attentional...

    Johannes Jacobus Fahrenfort in Spatial Learning and Attention Guidance (2020)

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