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    Measuring Interactions Between Tau and Aggregation Inducers with Single-Molecule Förster Resonance Energy Transfer

    手机体育投注平台 Tau is an intrinsically disordered protein implicated in the pathogenesis of Alzheimer’s disease and other neurodegenerative disorders. Here we describe the application of single-molecule Förster resonance ene...

    Sanjula P. Wickramasinghe, Elizabeth Rhoades in Intrinsically Disordered Proteins (2020)

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    A Guide for Protein–Protein Docking Using SwarmDock

    手机体育投注平台 Many of the biological functions of the cell are driven by protein–protein interactions. However, determining which proteins interact and exactly how they do so to enable their functions, remain major research...

    Iain H. Moal, Raphael A. G. Chaleil, Mieczyslaw Torchala in Protein Structure Prediction (2020)

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    Targeting an Intrinsically Disordered Protein by Covalent Modification

    手机体育投注平台 Intrinsically disordered proteins (IDPs) play important roles in the regulation of cellular function and in disease, and thus they represent an important group of therapeutic targets. Yet, members of this “dis...

    Hung Huy Nguyen, Péter Ábrányi-Balogh, László Petri in Intrinsically Disordered Proteins (2020)

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    Transfection of Small Noncoding RNAs into Arabidopsis thaliana Protoplasts

    手机体育投注平台 Polyethylene glycol transfection of plant protoplasts represents an efficient method to incorporate foreign DNA and study transient gene expression. Here, we describe an optimized protocol to deliver small non...

    Stéphanie Lalande, Marjorie Chery, Elodie Ubrig, Guillaume Hummel in RNA Tagging (2020)

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    Native Protein Mass Spectrometry

    In native mass spectrometry, non-covalent interactions are preserved in solution and through transfer to the gas phase. This technique can be used to characterize the composition, stoichiometry, and architectu...

    Timothy M. Allison, Mark T. Agasid in Protein Nanotechnology (2020)

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    Direct Binding Analysis Between C-Type Lectins and Glycans Using Immunoglobulin Receptor Fusion Proteins

    C-type lectins bind to carbohydrate structures in a Ca2+-dependent manner. Some transmembrane forms of lectins act as innate immune receptors and induce signal transduction pathways in macrophages and dendritic c...

    Miyuki Watanabe, Zakaria Omahdi, Sho Yamasaki in Lectin Purification and Analysis (2020)

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    Selection and Characterization of Anti-idiotypic Shark Antibody Domains

    The antibody repertoire of cartilaginous fish comprises an additional heavy-chain-only antibody isotype that is referred to as IgNAR (immunoglobulin novel antigen receptor). Its antigen-binding site consists o...

    Doreen Könning, Stefan Zielonka, Anna Kaempffe in Genotype Phenotype Coupling (2020)

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    Hybrid Silylated Peptides for the Design of Bio-functionalized Materials

    手机体育投注平台 Hybrid silylated peptides are useful starting compounds for the design of functionalized materials. Indeed, silylated peptides may react in soft conditions with other silylated species through Si–O–Si bonds, o...

    Titouan Montheil, Cécile Echalier, Jean Martinez in Peptide and Protein Engineering (2020)

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    Protein–Protein and Protein–Peptide Docking with ClusPro Server

    手机体育投注平台 The process of creating a model of the structure formed by a pair of interacting molecules is commonly referred to as docking. Protein docking is one of the most studied topics in computational and structural ...

    Andrey Alekseenko, Mikhail Ignatov, George Jones in Protein Structure Prediction (2020)

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    Recombinant Production of Monomeric Isotope-Enriched Aggregation-Prone Peptides: Polyglutamine Tracts and Beyond

    High solvent exposure of certain sequences located in intrinsically disordered regions (IDRs) may eventually lead to aggregation, as is the case for some low-complexity regions (LCRs) and short linear motifs (...

    Albert Escobedo, Giulio Chiesa, Xavier Salvatella in Intrinsically Disordered Proteins (2020)

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    Sialoglycovirology of Lectins: Sialyl Glycan Binding of Enveloped and Non-enveloped Viruses

    On the cell sur “face”, sialoglycoconjugates act as receptionists that have an important role in the first step of various cellular processes that bridge communication between the cell and its environment. Los...

    Nongluk Sriwilaijaroen, Yasuo Suzuki in Lectin Purification and Analysis (2020)

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    Peroxiredoxin Proteins as Building Blocks for Nanotechnology

    Peroxiredoxins are ubiquitous antioxidant proteins that exhibit a striking variety of quaternary structures, making them appealing building blocks with which nanoscale architectures are created for application...

    Frankie Conroy, N. Amy Yewdall in Protein Nanotechnology (2020)

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    ELISA-Based Methods to Detect and Quantify Norovirus Virus-Like Particle Attachment to Histo-Blood Group Antigens

    手机体育投注平台 Histo-blood group antigen (HBGA) recognition by norovirus (NoV) has been studied using various techniques. Enzyme-linked immunosorbent assays (ELISAs) using virus-like particles (VLPs) have enabled us to visua...

    Haruko Shirato in Lectin Purification and Analysis (2020)

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    In Vivo Histone Labeling Using Ultrafast trans-Splicing Inteins

    The development of expressed protein ligation (EPL) widened the scope of questions that could be addressed by mechanistic biochemistry. Protein trans-splicing (PTS) relies on the same basic chemical principles, b...

    Nicholas A. Prescott, Yael David in Expressed Protein Ligation (2020)

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    PNA-Encoded Synthesis (PES) and DNA Display of Small Molecule Libraries

    DNA-encoded library technologies have emerged as a powerful platform to rapidly screen for binders to a protein of interest. These technologies are underpinned by the ability to encode a rich diversity of smal...

    Jacques Saarbach, Sofia Barluenga, Nicolas Winssinger in Peptide Nucleic Acids (2020)

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    Reconstitution of Proteoliposomes for Phospholipid Scrambling and Nonselective Channel Assays

    Phospholipid scramblases catalyze the rapid trans-bilayer movement of lipids down their concentration gradients. This process is essential for numerous cellular signaling functions including cell fusion, blood...

    Maria E. Falzone, Alessio Accardi in Expression, Purification, and Structural B… (2020)

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    IDP-LZerD: Software for Modeling Disordered Protein Interactions

    Modeling the tertiary structure of protein–protein interaction complex has been well studied over many years, especially in the case where the structures of both binding partners are roughly the same before an...

    Charles Christoffer, Daisuke Kihara in Protein Structure Prediction (2020)

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    In-Cell NMR of Intrinsically Disordered Proteins in Mammalian Cells

    In-cell NMR enables structural insights at atomic resolution of proteins in their natural environment. To date, very few methods have been developed to study proteins by in-cell NMR in mammalian systems. Here ...

    Juan A. Gerez, Natalia C. Prymaczok, Roland Riek in Intrinsically Disordered Proteins (2020)

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    TRAP-SEQ of Eukaryotic Translatomes Applied to the Detection of Polysome-Associated Long Noncoding RNAs

    Translating ribosome affinity purification (TRAP) technology allows the isolation of polysomal complexes and the RNAs associated with at least one 80S ribosome. TRAP consists of the stabilization and affinity ...

    Soledad Traubenik, Flavio Blanco, María Eugenia Zanetti, Mauricio A. Reynoso in RNA Tagging (2020)

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    Molecular Dynamics Simulation of Proteins

    手机体育投注平台 Molecular dynamics simulations allow the conformational motion of a molecule such as a protein to be followed over time at atomic-level detail. Several choices need to be made prior to running a simulation, in...

    Thomas A. Collier, Thomas J. Piggot, Jane R. Allison in Protein Nanotechnology (2020)

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