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  1. No Access

    Protocol

    Design and Evaluation of Guide RNA Transcripts with a 3′-Terminal HDV Ribozyme to Enhance CRISPR-Based Gene Inactivation

    手机体育投注平台 The recently discovered clustered regularly interspaced short palindromic repeats (CRISPR)-Cpf1 system, now reclassified as Cas12a, is a DNA-editing platform analogous to the widely used CRISPR-Cas9 system. Th...

    Ben Berkhout, Zongliang Gao, Elena Herrera-Carrillo in Ribozymes (2021)

  2. No Access

    Protocol

    Design and Evaluation of AgoshRNAs with 3′-Terminal HDV Ribozymes to Enhance the Silencing Activity

    Since the first application of RNA interference (RNAi) in mammalian cells, the expression of short hairpin RNA (shRNA) molecules for targeted gene silencing has become a benchmark technology. Plasmid and viral...

    Ben Berkhout, Elena Herrera-Carrillo in Ribozymes (2021)

  3. Article

    Open Access

    A trip down memory lane with Retrovirology

    Monsef Benkirane, Ben Berkhout, Persephone Borrow, Ariberto Fassati in Retrovirology (2019)

  4. 手机体育投注平台 Article

    Open Access

    On the generation of the MSD-Ѱ class of defective HIV proviruses

    Antiretroviral therapy (ART) can effectively suppress ongoing HIV replication and block disease progression, but the infection is never cured due to the persistence of a small pool of latently infected cells h...

    Atze T. Das, Alexander O. Pasternak, Ben Berkhout in Retrovirology (2019)

  5. Article

    Open Access

    What do we measure when we measure cell-associated HIV RNA

    Cell-associated (CA) HIV RNA has received much attention in recent years as a surrogate measure of the efficiency of HIV latency reversion and because it may provide an estimate of the viral reservoir size. Th...

    Alexander O. Pasternak, Ben Berkhout in Retrovirology (2018)

  6. No Access

    Protocol

    Workup of Human Blood Samples for Deep Sequencing of HIV-1 Genomes

    We describe a detailed protocol for the manual workup of blood (plasma/serum) samples from individuals infected with the human immunodeficiency virus type 1 (HIV-1) for deep sequence analysis of the viral geno...

    Marion Cornelissen, Astrid Gall, Antoinette van der Kuyl in Viral Metagenomics (2018)

  7. 手机体育投注平台 Article

    Open Access

    The HIV-1 integrase-LEDGF allosteric inhibitor MUT-A: resistance profile, impairment of virus maturation and infectivity but without influence on RNA packaging or virus immunoreactivity

    HIV-1 Integrase (IN) interacts with the cellular co-factor LEDGF/p75 and tethers the HIV preintegration complex to the host genome enabling integration. Recently a new class of IN inhibitors was described, th...

    Céline Amadori, Yme Ubeles van der Velden, Damien Bonnard, Igor Orlov in Retrovirology (2017)

  8. Article

    Open Access

    HIV-1 tolerates changes in A-count in a small segment of the pol gene

    手机体育投注平台 The HIV-1 RNA genome has a biased nucleotide composition with a surplus of As. Several hypotheses have been put forward to explain this striking phenomenon, but the A-count of the HIV-1 genome has thus far not...

    Bep Klaver, Yme van der Velden, Formijn van Hemert in Retrovirology (2017)

  9. Article

    Open Access

    Professor Mark Wainberg

    Monsef Benkirane, Ben Berkhout, Persephone Borrow, Ariberto Fassati in Retrovirology (2017)

  10. 手机体育投注平台 Article

    Open Access

    Proceedings of the Frontiers of Retrovirology Conference 2016

    Oral presentations

    Irena Zurnic, Sylvia Hütter, Ute Lehmann, Nicole Stanke, Juliane Reh in Retrovirology (2016)

  11. Article

    Open Access

    Widespread hepatitis B virus genotype G (HBV-G) infection during the early years of the HIV epidemic in the Netherlands among men who have sex with men

    Hepatitis B virus (HBV) variants belong to different genotypes, A-J, whose worldwide distribution is linked with geography, probably because viral spread was associated with ancient human migrations. HBV genot...

    Marion Cornelissen, Fokla Zorgdrager, Sylvia M. Bruisten in BMC Infectious Diseases (2016)

  12. Article

    Open Access

    Nucleotide composition of the Zika virus RNA genome and its codon usage

    RNA viruses have genomes with a distinct nucleotide composition and codon usage. We present the global characteristics of the RNA genome of Zika virus (ZIKV), an emerging pathogen within the Flavivirus genus. ...

    Formijn van Hemert, Ben Berkhout in Virology Journal (2016)

  13. Article

    Open Access

    CRISPR/Cas9: a double-edged sword when used to combat HIV infection

    Chen Liang, Mark A. Wainberg, Atze T. Das, Ben Berkhout in Retrovirology (2016)

  14. 手机体育投注平台 Article

    Open Access

    HIV-1 transcriptional silencing caused by TRIM22 inhibition of Sp1 binding to the viral promoter

    手机体育投注平台 Intracellular defense proteins, also referred to as restriction factors, are capable of interfering with different steps of the viral life cycle. Among these, we have shown that Tripartite motif 22 (TRIM22) su...

    Filippo Turrini, Sara Marelli, Anna Kajaste-Rudnitski, Marina Lusic in Retrovirology (2015)

  15. Article

    Open Access

    On the primer binding site mutation that appears and disappears during HIV and SIV replication

    A recent study by Fennessey et al. (Retrovirology 12:49, 2015) described the optimization of a popular SIV clone by removal of four suboptimal point mutations. One of these mutations is present in a non-coding pa...

    Ben Berkhout, Atze T. Das in Retrovirology (2015)

  16. Article

    Open Access

    ABX464: a good drug candidate instead of a magic bullet

    Despite the significant number of antiviral drugs that are currently available in the clinics of developed countries, none of these affect the production stage of HIV-1 replication, more specifically the proce...

    Ben Berkhout, Yme U van der Velden in Retrovirology (2015)

  17. No Access手机体育投注平台

    手机体育投注平台 Book

  18. No Access手机体育投注平台

    Chapter

    HIV Infection and Adipose Tissue Resident Stem Cells: Their Involvement in Pathology and Treatment

    手机体育投注平台 The HIV reservoir creates a true challenge for eradicating the virus from infected patients. Current Highly Active Antiretroviral Therapy (HAART) is very effective in controlling active viral replication in th...

    Upal Roy, Ben Berkhout in Stem Cells and Cancer Stem Cells, Volume 13 (2015)

  19. 手机体育投注平台 Article

    Open Access

    Obituary: Axel Rethwilm (1959–2014)

    手机体育投注平台 No abstract.

    Ben Berkhout, Jochen Bodem, Otto Erlwein, Ottmar Herchenröder in Retrovirology (2014)

  20. No Access

    Protocol

    HIV-1-Based Lentiviral Vectors

    Numerous viral vectors have been developed for the delivery of transgenes to specific target cells. For persistent transgene expression, vectors based on retroviruses are attractive delivery vehicles because o...

    Ying Poi Liu, Ben Berkhout in Human Retroviruses (2014)

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