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    Protocol

    Substitutional RNA Editing in Plant Organelles

    RNA editing by cytidine (C) to uridine (U) conversions frequently occurs in land plant mitochondria and plastids. Target cytidines are specifically recognized by nuclear-encoded pentatricopeptide repeat (PPR) ...

    Mizuho Ichinose, Mamoru Sugita in RNA Editing (2021)

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    手机体育投注平台 Article

    The 5′ untranslated region of the rbp1 mRNA is required for translation of its mRNA under low temperatures in the cyanobacterium Synechococcus elongatus

    The unicellular cyanobacterium Synechococcus elongatus has three RNA-binding protein (Rbp) genes, rbp1, rbp2 and rbp3. The rbp1 gene was upregulated by cold treatment while rbp2 and rbp3 expression decreased rema...

    Rie Hayashi, Chieko Sugita, Mamoru Sugita in Archives of Microbiology (2017)

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    手机体育投注平台 Article

    Plastid genome sequences of Gymnochlora stellata, Lotharella vacuolata, and Partenskyella glossopodia reveal remarkable structural conservation among chlorarachniophyte species

    Chlorarachniophyte algae have complex plastids acquired by the uptake of a green algal endosymbiont, and this event is called secondary endosymbiosis. Interestingly, the plastids possess a relict endosymbiont ...

    Shigekatsu Suzuki, Yoshihisa Hirakawa, Rumiko Kofuji in Journal of Plant Research (2016)

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    Open Access

    DipM is required for peptidoglycan hydrolysis during chloroplast division

    手机体育投注平台 Chloroplasts have evolved from a cyanobacterial endosymbiont and their continuity has been maintained over time by chloroplast division, a process which is performed by the constriction of a ring-like division...

    Shin-ya Miyagishima, Yukihiro Kabeya, Chieko Sugita, Mamoru Sugita in BMC Plant Biology (2014)

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    手机体育投注平台 Protocol

    Plastid Transformation in Physcomitrella patens

    The moss Physcomitrella patens performs efficient homologous recombination in both the nucleus and plastid enabling the study of individual gene function by generating precise inactivation or modification of gene...

    Mamoru Sugita in Chloroplast Biotechnology (2014)

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    Chapter

    Dynamic Features of Plastid Genome and Its Transcriptional Control in Plastid Development

    手机体育投注平台 Land plants usually have different types of plastids, e.g. etioplasts, chloroplasts, amyloplasts, and chromoplasts. Although identical copies of the plastid genome are present in all plastid types, the level a...

    Kengo Kanamaru, Mamoru Sugita in Plastid Development in Leaves during Growth and Senescence (2013)

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    Article

    Complete nucleotide sequence of the freshwater unicellular cyanobacterium Synechococcus elongatus PCC 6301 chromosome: gene content and organization

    The entire genome of the unicellular cyanobacterium Synechococcus elongatus PCC 6301 (formerly Anacystis nidulans Berkeley strain 6301) was sequenced. The genome consisted of a circular chromosome 2,696,255 bp lo...

    Chieko Sugita, Koretsugu Ogata, Masamitsu Shikata in Photosynthesis Research (2007)

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    手机体育投注平台 Article

    Chloroplast transformation reveals that tobacco ycf5 is involved in photosynthesis

    Hypothetical chloroplast reading frame 5 (ycf5) is encoded by the chloroplast genome and potentially specifies a protein of 313 amino acids in tobacco. A northern blot analysis showed that ycf5 transcripts accumu...

    Kayo Tsuruya, Makoto Suzuki, Wojciech Plader, Chieko Sugita in Acta Physiologiae Plantarum (2006)

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    Chapter

    Transcriptional and Translational Regulation of Photosystem II Gene Expression

    The plastid gene expression system employs a highly developed transcription and translation system that is an elaborated prokaryotic and eukaryotic chimera. Photosystem II (PS II) gene expression is regulated ...

    Kenichi Yamaguchi, Stephen P. Mayfield, Mamoru Sugita in Photosystem II (2005)

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    Article

    Molecular cloning, expression and characterization of three distinctive genes encoding methionine aminopeptidases in cyanobacterium Synechocystis sp. strain PCC6803

    Methionine aminopeptidase, known to be encoded by single genes in prokaryotes, is a cobalt-dependent enzyme that catalyzes the removal of N-terminal methionine residues from nascent polypeptides. Three ORFs en...

    Anelia Atanassova, Mamoru Sugita, Masahiro Sugiura in Archives of Microbiology (2003)

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    手机体育投注平台 Chapter

    Structure and Expression of LHCB Genes from Nicotiana Sylvestris

    Light harvesting chlorophyll a/b-binding proteins, Lhca and Lhcb, are major components of antenna complexes in PSI and PSII, respectively. Lhca and Lhcb proteins are encoded by the nuclear genes, synthesized as p...

    Keiko Hasegawa, Yasushi Yukawa, Miyoko Niwa in Photosynthesis: Mechanisms and Effects (1998)

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    Chapter

    The Function of the Cyanobacterial RNA-Binding Proteins

    Posttranscriptional and translational regulation of gene expression in cyanobacteria have recently been reported (1-3). The different stability of mRNAs is suggested to have an important role for the regulatio...

    Michinori Mutsuda, Tetsuro Hirose in Photosynthesis: Mechanisms and Effects (1998)

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    手机体育投注平台 Chapter

    Characterization of Ribonucleoproteins from the Tobacco Chloroplast

    Tobacco chloroplasts contain five distinct RNA-binding proteins, cp28 to cp33, which consist of an acidic N-terminal domain and two RNA-binding domains (1, 2). Spinach 28 kDa protein, 28RNP, similar to the tob...

    Takahiro Nakamura, Masaru Ohta, Mamoru Sugita in Photosynthesis: Mechanisms and Effects (1998)

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    Chapter

    In Vitro Transcription Analysis of the rbcS Gene

    Photosynthetic gene expression is a most significant theme of plant molecular biology. Until now, many researcher have approached to make clear this problem from different aspects. Especially light-dependent g...

    Yasushi Yukawa, Mamoru Sugita, Masahiro Sugiura in Photosynthesis: Mechanisms and Effects (1998)

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    Article

    Regulation of gene expression in chloroplasts of higher plants

    手机体育投注平台 Chloroplasts contain their own genetic system which has a number of prokaryotic as well as some eukaryotic features. Most chloroplast genes of higher plants are organized in clusters and are cotranscribed as p...

    Mamoru Sugita, Masahiro Sugiura in Plant Molecular Biology (1996)

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    Article

    Physical and gene maps of the unicellular cyanobacterium Synechococcus sp. strain PCC6301 genome

    A physical map of the unicellular cyanobacterium Synechococcus sp. strain PCC6301 genome has been constructed with restriction endonucleases PmeI, SwaI, and an intron-encoded endonuclease I-CeuI. The estimated si...

    Takakazu Kaneko, Tohru Matsubayashi, Mamoru Sugita in Plant Molecular Biology (1996)

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    Article

    Isolation of a novel RNA-binding protein and its association with a large ribonucleoprotein particle present in the nucleoplasm of tobacco cells

    A cDNA encoding a protein with a consensus sequence-type RNA-binding domain (CS-RBD) has been isolated from a Nicotiana sylvestris cDNA library. The deduced protein (designated ‘RZ-1’) contains CS-RBD in its N-te...

    Shigeru Hanano, Mamoru Sugita, Masahiro Sugiura in Plant Molecular Biology (1996)

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    手机体育投注平台 Chapter

    Regulation of gene expression in chloroplasts of higher plants

    手机体育投注平台 Chloroplasts contain their own genetic system which has a number of prokaryotic as well as some eukaryotic features. Most chloroplast genes of higher plants are organized in clusters and are cotranscribed as p...

    Mamoru Sugita in Post-Transcriptional Control of Gene Expression in Plants (1996)

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    手机体育投注平台 Article

    Transient expression of β-glucuronidase in plastids of various plant cells and tissues delivered by a pneumatic particle gun

    Chloroplast expression plasmids pTRBCL-GUS (tobaccorbcL promoter-gusA-tobaccorbcL terminator) and pHHU3004 (spinach ‘x gene’ promoter-gusA-spinachrbcL手机体育投注平台 terminator) and a control nuclear expression plasmid pBI221 (...

    Motoaki Seki, Naoki Shigemoto, Mamoru Sugita, Masahiro Sugiura in Journal of Plant Research (1995)

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    Article

    Structure and expression of the gene encoding ribosomal protein S1 from the cyanobacterium Synechococcus sp. strain PCC 6301: striking sequence similarity to the chloroplast ribosomal protein CS1

    We isolated a 38 kDa ssDNA-binding protein from the unicellular cyanobacterium Synechococcus手机体育投注平台 sp. strain PCC 6301 and determined its N-terminal amino acid sequence. A genomic clone encoding the 38 kDa protein was ...

    Mamoru Sugita, Chieko Sugita, Masahiro Sugiura in Molecular and General Genetics MGG (1995)

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