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    手机体育投注平台 Protocol

    Inverse RNA Folding Workflow to Design and Test Ribozymes that Include Pseudoknots

    手机体育投注平台 Ribozymes are RNAs that catalyze reactions. They occur in nature, and can also be evolved in vitro to catalyze novel reactions. This chapter provides detailed protocols for using inverse folding software to de...

    Mohammad Kayedkhordeh, Ryota Yamagami, Philip C. Bevilacqua, David H. Mathews in Ribozymes (2021)

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    Open Access

    mRNA structural elements immediately upstream of the start codon dictate dependence upon eIF4A helicase activity

    The RNA helicase eIF4A1 is a key component of the translation initiation machinery and is required for the translation of many pro-oncogenic mRNAs. There is increasing interest in targeting eIF4A1 therapeutica...

    Joseph A. Waldron, David C. Tack, Laura E. Ritchey, Sarah L. Gillen in Genome Biology (2019)

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    Protocol

    In Vivo Genome-Wide RNA Structure Probing with Structure-seq

    In vivo genome-wide RNA structure probing provides a global view of RNA structure as it occurs in the cell and can assist in elucidating important functional aspects of RNA structure. Structure-seq2 provides h...

    Laura E. Ritchey, Zhao Su, Sarah M. Assmann in Plant Long Non-Coding RNAs (2019)

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    Chapter

    An Integrated Picture of HDV Ribozyme Catalysis

    The hepatitis delta virus (HDV) ribozyme, a small self-cleaving RNA originally identified in the human pathogen HDV, has been found to be broadly dispersed throughout life. In this article, we describe an inte...

    Barbara L. Golden, Sharon Hammes-Schiffer, Paul R. Carey in Biophysics of RNA Folding (2013)

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    手机体育投注平台 Protocol

    Analysis of RNA Folding and Ligand Binding by Conventional and High-Throughput Calorimetry

    Noncoding RNAs serve myriad functions in the cell, but their biophysical properties are not well understood. Calorimetry offers direct and label-free means for characterizing the ligand-binding and thermostabi...

    Joshua E. Sokoloski, Philip C. Bevilacqua in Bacterial Regulatory RNA (2012)

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    手机体育投注平台 Protocol

    Mechanistic Analysis of the Hepatitis Delta Virus (HDV) Ribozyme: Methods for RNA Preparation, Structure Mapping, Solvent Isotope Effects, and Co-transcriptional Cleavage

    手机体育投注平台 Small ribozymes such as the hairpin, hammerhead, VS, glm S, and hepatitis delta virus (HDV) are self-cleaving RNAs that are typically characterized by kinetics and structural methods. Working with these RNAs r...

    Durga M. Chadalavada, Andrea L. Cerrone-Szakal, Jennifer L. Wilcox in Ribozymes (2012)